Methods from the Breeden Lab
 

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General - methods SDS-Polyacrylamide gels silver staining western blotting mRNA purification northern blot differential display of RNAs chromatin IP (CHIP assay) coupling antibodies to protein A/G DNaseI footprinting S1 Assay Using Oligo Probes

Yeast - methods yeast transformation RNA miniprep genomic DNA miniprep chromosomal DNA prep ß-GAL filter assay FACS on yeasts FACS - Sytox on yeasts yeast colony PCR Yeast gene deletion/disruption

E.coli - methods plasmid DNA miniprep competent E.coli cells E. coli transformation

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Last update: 3/13/02
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