Zeiss LSM 510 META spectral confocal and two photon microscope
Confocal microscopy uses optical methods to remove the out-of-focus component of fluorescence microscopy images. The system is highly automated, and allows the imaging of large fields of view (tiling) and multi-point visiting. Finally, the system includes devices for temperature control, and is therefore well-suited for the imaging of live cells or embryos. A variety of top-of-the-line optics are available for most imaging needs.
Our state-of-the-art Zeiss LSM 510 META confocal microscope offers three lasers in the visible range (456/488, 543, and 633nm) for fluorescence excitation, and is capable of imaging up to eight fluorescent channels. The system includes a Coherent Chameleon tunable near IR laser for the excitation of UV-excited dyes such as DAPI, and for multiphoton imaging. Two photon offers benefits for the imaging of thick samples, and for long-term imaging of live samples. Specialized techniques such as Fluorescence Recovery after Photobleaching (FRAP), Fluorescence Resonance Energy Transfer (FRET), and Calcium ratio imaging are also possible. See the Zeiss LSM 510 manual (PDF)
Leica TCS SP confocal microscope
The Leica TCS SP features proprietary filterless detector optics with a 3 laser configuration (488/561/633nm). The instrument is equipped with a filterless spectral detector that provides the flexibility of freely defining wavelength bands for each channel, allowing the user to maximize emission sensitivity and separate closely spaced fluorescent dyes.
Applied Precision, Inc. Deltavision RT Wide-field Deconvolution Microscope
The Deltavision system uses Fourier transform mathematics to remap the out-of-focus components of three dimensional data sets. By using mathematical methods, this system is able to achieve superior sensitivity versus confocal methods while producing higher resolution images. In addition, since the system is not laser based, a wide range of excitation and emission wavelengths can be employed (350nm to >700nm) and considerably less photo damage occurs. The increase in sensitivity permits this system to be used for time-lapse and 4D (3-D + time) experiments.
Two filter sets are available: standard 4-band set (DAPI, FITC, Rhodamine, CY5), and Live-Cell Filter set (GFP, CFP, YFP and DsRed). A Bioptechs temperature-controlled chamber is available for live cell experiments on the DeltaVision.
Nikon Eclipse E800
For basic fluorescence imaging needs, this easy to use fixed-stage microscope allows the collection of images in the DAPI, FITC and Rhodamine channels in fluorescence mode, as well as Differential Interference Contrast (DIC) microscopy. See the Nikon E800 tutorial (PDF, 9.9KB).
Zeiss M2Bio fluorescence stereomicroscope
The M2Bio fluorescence stereomicroscope ("dissecting" microscope) assembled by Kramer Scientific on a Zeiss Stemi V6 stereomicroscope platform is a versatile imaging instrument. The M2Bio is equipped with fluorescence filters for BFP, CFP, GFP (FITC), YFP and DsRed (TRITC), and with a CCD camera for digital multi-channnel and time lapse image acquisition. It is mainly used as a support microscope to sort and/or identify fluorescent samples for imaging on our high-end microscopes, but can also be used on its own.
Typhoon Trio Multi-mode imager.
The Typhoon Trio is a versatile instrument for the imaging of radioactive, fluorescent and chemiluminescent samples. The instrument can scan gels, blots, multi-well plates, and various other samples, at resolutions up to 50 microns. Images can be analyzed and quantitated with ImageQuant, or with the public domain ImageJ software package (Imaging Software). Most common applications include the imaging and quantitation of radioactively labeled samples (such as Northern blots and Southern blots), and the imaging of fluorescent samples such as fluorescent Western Blots and DNA gels. The Trio has three lasers, for optimal excitation of a variety of dyes. The more limited and older Typhoon 8600 is available as a back-up.
Li-Cor Odyssey infra-red imaging system.
The Odyssey system is a sensitive far red/infrared two-color protein and nucleic acid fluorescence detection system for gels, blots, 96-well plates and a variety of other applications, including whole animal imaging. The instrument has two detection channels at 700 and 800 nm, and is especially well-suited for two-color Western blot assays, where it provides a convenient, economic, and more quantitative alternative to chemiluminescence. Data analysis can be performed with Odyssey software (please request one copy from the Resource if needed), or with public domain software such as ImageJ (see Imaging Software).
Epson flat-bed scanner.
A professional Epson flat-bed scanner is also available in the facility, for the scanning, digitization, and quantitation of photographic images of gels and a variety of other samples. The flat-bed scanner can image a sample up to 8 x 10 inches at up to 1200 dpi. Scans are acquired via Photoshop software, and can further be quantitated with ImageJ or ImageQuant.
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