Techniques such as SILAC, iTRAQ, ICAT, and AQUA are available for quantifying proteins and peptides by mass spectrometry. All of the techniques include some form of stable isotope (e.g. carbon-13) incorporation into proteins that is detected by the mass spectrometer and used for quantification. Each quantification technique has its own strengths, limitations and requirements. The Proteomics Facility should be consulted during the design of proteomics experiments to ensure the appropriate technique is selected.
Sample preparation involves SDS-PAGE gel electrophoresis, digestion, and desalting of gel slices. Investigators can take their samples through the complete preparation, or the proteomics staff can assist with some steps. If you choose to prepare your own gel samples, see our Tips for SDS-PAGE Gel Handling
If you are using a FHCRC project id for billing, please fill out the Internal Submission Form and submit with your sample.
If billing is to be submitted to the University of Washington or other external institution, fill out the External Submission Form and submit with your sample.
Data generated within the resource are transferred through automated pipelines to systems supported by the Hutchinson Center's Research Computing Support shared resource. Data is transferred to the user's 'fred' account, in the researcher's proteomics folder.
The incorporation of quantification results into CPAS is an on-going project. Please contact the Proteomics Facility for assistance with viewing quantification results.
